Volume: 4 Issue 3 [March 2021]

Article:Growth Inhibitory Effects of Vitamin K2 (Menaquinones- MK4) on Bladder Carcinoma and Prostate Carcinoma Cells Via Two Possible Pathways of Apoptosis Implicating PUMA, BAX and Cleaved-PARP

Author: Samira Zohra Midoun , Ling Hong

Volume: Vol 4, Issue 3; March 2021

DOI: North American Academic Research, 4(3)192-211, Mar 2021, https://doi.org/10.5281/zenodo.4642494

Abstract: Ubiad1 is a tumor suppressor gene ubiquitously expressed in normal human tissues and its protein harbors a prenyltransferase functional domain conserved throughout the evolution. The ability of human UBIAD1 enzyme to synthesize menaquinone-4 (MK-4) rescues mitochondrial dysfunction in Drosophila and effectively initiates apoptosis in various types of tumors through a caspase-dependent pathway. The objective is to study menaquinone-4-induced apoptosis signaling pathway and to investigate its anti-tumor activity in human prostate (PC-3, LNCaP) and bladder (T24, J82) tumor cell lines, an approach that has not been undertaken yet. Cell viability of cancerous cells pre-treated with MK-4 showed a significant decrease in dose- and timedependent manner. Moreover, flow cytometry detected apoptotic cells after treatment with the vitamin. Taken together, these observations suggest that MK-4 could inhibit cell growth by promoting apoptosis in prostate and bladder carcinoma cells via different-mediated signal pathways, making it a potential therapeutic molecule for the prevention and the cure of cancers.

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